NAD+ and NADH regulate an ATP-dependent kinase that

NAD+ and NADH regulate an ATP-dependent kinase that ABSTRACT Crude extracts of Escherichia coil contain a protein kinase, El-K, that phosphorylates enzyme I (El) of the phosphoenolpyruvate:glycose phosphotransferase system (PTS). Phosphorylation occurs at the active site hsdine residue. The activity of EI-K was lost during purifiation. However, kinase activity was restored by adding NAD+ or…

Nadh_Sensor of blood flow need

Nadh_Sensor of blood flow need ABSTRACT The sensor for blood-flow need with neural activity and exercise is not known. We tested the hypothesis that accumulation of electrons in cytosolic free nicotinamide adenine dinucleotide (NAD) activates redox signaling pathways to augment blood flow. NAD is the primary carrier of electrons from glucose and lactate for ATP…

NADH_NAD redox state of cytoplasmic glycolytic

NADH_NAD redox state of cytoplasmic glycolytic Barron, John T., Liping Gu, and Joseph E. Parrillo. NADH/NAD redox state of cytoplasmic glycolytic compartments in vascular smooth muscle. Am J Physiol Heart Circ Physiol 279: H2872–H2878, 2000.—The cytoplasmic NADH/ NAD redox potential affects energy metabolism and contractile reactivity of vascular smooth muscle. NADH/NAD redox state in the…

NADH supplementation decreases pinacidil-primed

NADH supplementation decreases pinacidil-primed Abstract The aim of this study was to investigate the effect of NADH-supplementation on the metabolic condition of isolated guinea pig ventricular cardiomyocytes using the pinacidil-primed IK(ATp) as an indicator of subsarcolemmal ATP concentration. Membrane currents were studied using the patch-clamp technique in the whole-cell recording mode at 36-37oC. Under physiological conditions (4.3 mM ATP…

NADH in Cancer Prevention

NADH in Cancer Prevention All living cells require energy to stay alive. Without energy, a cell dies because the energy production represents the essential prerequisites for every living cell. How is energy produced in the cell? NADH reacts with oxygen to produce in a cascade of biochemical reactions producing water and energy. This energy is…

nadh and calcium

nadh and calcium As an important second messenger, calcium triggers a broad range of energy-consuming cellular reactions like contraction, secretion and gene expression (Clapham, 1995; Berridge et al. 1998; Brini & Carafoli, 2000). To match the increased energy demand, calcium simultaneously upregulates the rates of NADH production and ATP synthesis (McCormack et al. 1990; Hajnoczky…

NAD and NADH in brain functions, brain diseases and brain aging

NAD and NADH in brain functions, brain diseases and brain aging 1. ABSTRACT Numerous studies have suggested that NAD+ and NADH mediate multiple major biological processes, including calcium homeostasis, energy metabolism, mitochondrial functions, cell death and aging. In particular, NAD+ and NADH have emerged as novel, fundamental regulators of calcium homeostasis. It appears that most…

Energy dependence of restitution in the gastric mucosa

Energy dependence of restitution in the gastric mucosa Cheng, Aaron M., Sarah W. Morrison, David X. Yang, and Susan J. Hagen. Energy dependence of restitution in the gastric mucosa. Am J Physiol Cell Physiol 281: C430–C438, 2001.—Rapid epithelial repair (restitution) after injury is required to maintain barrier function of the gastrointestinal mucosa and skin and…

Effectiveness of nadh in alleviating effects of acute sleep deprivation

Effectiveness_of_nadh_in Alleviating_Effects_of_Acute_Sleep_Deprivation INTRODUCTION Chronic sleep deprivation is very common in the general population, leading to well-described difficulties in concentration and cognitive performance and increases in irritability and malaise, among other symptoms (1). Acute sleep deprivation, when an individual must forgo most or all of a night’s sleep can also lead to immediate daytime consequences. Ta…