Electrostatic effects of bound NADH and NAD’ on ionizing groups in liver alcohol dehydrogenase A simple point charge model has been used to estimate the effect of electrostatic interactions of bound NADH and NAD’ on ionizing groups in liver alcohol dehydrogenase. The results provide clear evidence that the muchdiscussed effects of pH on coenzyme binding…
Einfluss des NAD(H) Pools Abstract The aim of this work was to limit the intracellular NAD turnover and to increase the intracellular NAD concentration in Escherichia coli in order to construct a strain characterized by higher productivity and improved long-term stability in the reductive wholecell biotransformation. To this end, first the genes yrfE and yjaD…
Einfluss des Kofaktors NAD_NADH der S-Adenosylhomocystein-Hydrolase auf die Adenosinbindung Einleitung Dass dieses Enzym eines der konserviertesten Enzyme ist, zeigt ein Vergleich der Aminosäuresequenz des menschlichen Enzyms mit dem der Ratte. Die Aminosäuresequenz der SAH-Hydrolase der Ratte stimmt zu 97% mit der des menschlichen Enzyms, und zu 52% mit der Sequenz des Enzyms bei Plasmodium falciparum…
Effects of NADH Supplementation
Effects of NADH on Dopamine Release in Rat Striatum INTRODUCTION Parkinson’s disease (PD) is characterized by a progressive degeneration of dopaminergic terminals in the nigrostriatal pathway. Postmortem analysis has shown that tyrosine hydroxylase (TH), the rate-limiting enzyme of DA synthesis, is depleted in the striatum of patients with this disorder (McGeer et al., 1971). TH…
Effects of Extracellular NADH on Human Tumor Cell Proliferation 1999
Effectiveness_of_nadh_in Alleviating_Effects_of_Acute_Sleep_Deprivation INTRODUCTION Chronic sleep deprivation is very common in the general population, leading to well-described difficulties in concentration and cognitive performance and increases in irritability and malaise, among other symptoms (1). Acute sleep deprivation, when an individual must forgo most or all of a night’s sleep can also lead to immediate daytime consequences. Ta…
Direct transfer of NADH ABSTRACT Following the criticism by Chock and Gutfreund [Chock, P. B. & Gutfreund, H. (1988)Proc. Nall. Acad. Sci. USA 85, 8870-8874], that our proposal of direct transfer of NADH between glycerol-3-phosphate dehydrogenase (aglycerol phosphate dehydrogenase, a-GDH; EC 1.1.1.8) and L-lactate dehydrogenase (LDH; EC 1.1.1.27) was based on a misinterpretation of the…
Digestion and Absoption of NAD by the Small Intestine of the Rat ABSTRACT A number of preparations of varying complexity have been used in an effort to elucidate the reactions by which NAD is hydrolyzed to nicotinamide during intestinal digestion. NAD labeled with 14Cin the adenine or pyridine moiety was the substrate used with perfused…
Differential binding of NAD+ and NADH Carboxyl-terminal binding protein (CtBP) is a transcriptional corepressor originally identified through its ability to interact with adenovirus E1A. The finding that CtBP–E1A interactions were regulated by the nicotinamide adeninine dinucleotides NAD and NADH raised the possibility that CtBP could serve as a nuclear redox sensor. This model requires differential…