Ca2 NADPH and membrane potential

Ca2+, NAD(P)H and membrane potential

The present studywas undertaken to determine the main metabolic
secretory signals generated by the mitochondrial substrate MeS
(methyl succinate) compared with glucose in mouse and rat islets
and to understand the differences. Glycolysis and mitochondrial
metabolism both have key roles in the stimulation of insulin
secretion by glucose. Both fuels elicited comparable oscillatory
patterns of Ca2+ and changes in plasma and mitochondrial
membrane potential in rat islet cells and clonal pancreatic β-
cells (INS-1). Saturation of the Ca2+ signal occurred between 5
and 6 mM MeS, while secretion reached its maximum at 15 mM,
suggesting operation of a KATP-channel-independent pathway.
Additional responses to MeS and glucose included elevated
NAD(P)H autofluorescence in INS-1 cells and islets and increases
in assayed NADH and NADPH and the ATP/ADP ratio…

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